A. All required equipment, supplies, and materials for the programme are procured for the PICTs, and necessary arrangements made prior to country implementation.
B. Country implementation is carried out by a team from SPC consisting of an epidemiologist and a laboratory specialist. Implementation activities include: determination of in-country sentinel sites and presentations of the programme to all country stakeholders (clinicians, lab technical staff, epidemiologists). The presentations focus on appropriate case definition and patient selection, in-country testing methods and test referral procedures, and management of data and results for surveillance mechanisms. (see 1, 2)
C. Implementation activities also include: training of the lab technical staff on all aspects of the immunofluorescent staining procedures and microscopy techniques, including appropriate quality assurance mechanisms for the testing. (see 1, 3, 4, 5, 6, 7, 8)
D. After initial performance of the lab testing procedures, a second intensive training, for the technical staff, is scheduled at one of the laboratory training institutions, WHO-CC or ESR. > Read the article in Inform'ACTION 22 This is to re-enforce the laboratory technical procedures and also as an opportunity for the trainees to troubleshoot problems or situations experienced in their initial testing performance.
E. All in-country stakeholders are also trained on the mechanisms of data collection and reporting of results in a timely manner to the appropriate individuals, so as to complete the surveillance aspects of the programme.
Transmission form for influenza testing (pdf, 55 KB)
Specimen collection instructions (pdf, 33 KB)
Immunofluorescent staining for respiratory virus detection: influenza A, influenza B and respiratory syncytial virus (pdf, 126 KB)
Fluorescent microscope operation (pdf, 17 KB)
Microscopy result worksheet
(pdf, 20 KB)
Influenza fluorescent strain quality control record (pdf, 29 KB)
Standard Operating Procedure: External Quality Assurance Program for the CDC lab-based influenza surveillance (pdf, 38 KB)
CDC Flu project EQA report form (pdf, 23 KB)